Hotspot

trnK-TTT-rps16

LSC · 4167-4886 · 720 bp

Back to Leonurus

Pi 0.00185
MarkerSeek score 59.90
Primer available yes
Label trnK-TTT-rps16

Feature Metadata

label_name
trnK-TTT-rps16
feature_type
igs
region
LSC
length_bp
720
variable_sites
2
pi
0.00185
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-TTT-rps16_p1 CCCTTTCAGGATCAGTCGTG TTTCCTTGAAAGGGGTGCTC 55.0 50.0 58.0 58.0 942-945 bp 1.0 80.0
trnK-TTT-rps16_p2 CCTTTCAGGATCAGTCGTGG TTTCCTTGAAAGGGGTGCTC 55.0 50.0 58.0 58.0 941-944 bp 1.0 80.0
trnK-TTT-rps16_p3 TCCCTTTCAGGATCAGTCGT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.1 58.0 943-946 bp 1.0 79.6
trnK-TTT-rps16_p4 TCAGGATCAGTCGTGGTCTT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.1 58.0 937-940 bp 1.0 79.5
trnK-TTT-rps16_p5 TTCAGGATCAGTCGTGGTCT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.1 58.0 938-941 bp 1.0 79.5