Hotspot

ycf3

LSC · 41801-43740 · 1940 bp

Back to Leontopodium

Pi 0.000529

MarkerSeek score 49.30

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1940
variable_sites: 12
pi: 0.000529
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGATGGGGCCAACAAACAAT	TCCCGCGAGAAGTAATGAGA	45.0	50.0	58.3	58.2	2098-2106 bp	1.0	76.5
ycf3_p2	TGATGGGGCCAACAAACAAT	GGATCATTTTCCCGCGAGAA	45.0	50.0	58.3	58.3	2107-2115 bp	1.0	75.8
ycf3_p3	TGATGGGGCCAACAAACAAT	ATCATTTTCCCGCGAGAAGT	45.0	45.0	58.3	57.6	2105-2113 bp	1.0	75.2
ycf3_p4	TGATGGGGCCAACAAACAAT	CCCGCGAGAAGTAATGAGAA	45.0	50.0	58.3	57.4	2097-2105 bp	1.0	73.8
ycf3_p5	TGATGGGGCCAACAAACAAT	TTCCCGCGAGAAGTAATGAG	45.0	50.0	58.3	57.4	2099-2107 bp	1.0	73.8