Hotspot

ndhC-trnV-UAC

LSC · 49666-50854 · 1189 bp

Back to Leontopodium

Pi 0.000906

MarkerSeek score 47.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1189
variable_sites: 9
pi: 0.000906
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTTGCTACTCGGAGCTA	GAGAAGGTCTACGGTTCGAG	50.0	55.0	57.9	57.2	1334-1343 bp	1.0	73.0
ndhC-trnV-UAC_p2	TCCTTTGCTACTCGGAGCTA	CGAGAAGGTCTACGGTTCGA	50.0	55.0	57.9	58.9	1335-1344 bp	1.0	72.3
ndhC-trnV-UAC_p3	ACTCGGAGCTAAAAATCCCG	GAGAAGGTCTACGGTTCGAG	50.0	55.0	57.7	57.2	1325-1334 bp	1.0	71.7
ndhC-trnV-UAC_p4	TTCTCCGGTCCTTTGCTACT	GAGAAGGTCTACGGTTCGAG	50.0	55.0	58.4	57.2	1342-1351 bp	1.0	71.3
ndhC-trnV-UAC_p5	ACTCGGAGCTAAAAATCCCG	CGAGAAGGTCTACGGTTCGA	50.0	55.0	57.7	58.9	1326-1335 bp	1.0	70.9