Hotspot

psaA-ycf3

LSC · 41037-41800 · 764 bp

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Pi 0.000955

MarkerSeek score 47.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 8
pi: 0.000955
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AGAAATGACCCGGTTTAGCC	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	953-957 bp	1.0	78.0
psaA-ycf3_p2	AGAAATGACCCGGTTTAGCC	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	955-959 bp	1.0	78.0
psaA-ycf3_p3	CCCATGCCTCGAATGAAGTT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.2	58.3	935-939 bp	1.0	77.1
psaA-ycf3_p4	CCCATGCCTCGAATGAAGTT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.2	58.3	937-941 bp	1.0	77.1
psaA-ycf3_p5	GTTTAGCCCATGCCTCGAAT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.3	58.3	941-945 bp	1.0	76.5