Hotspot

trnG-UCC-psbD

LSC · 30360-31992 · 1633 bp

Back to Leontopodium

Pi 0.00075

MarkerSeek score 48.70

Primer available yes

Label trnG-UCC-psbD

Feature Metadata

label_name: trnG-UCC-psbD
feature_type: igs
region: LSC
length_bp: 1633
variable_sites: 11
pi: 0.00075
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbD_p1	AATCAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	45.0	55.0	57.0	58.0	1769-1796 bp	1.0	74.4
trnG-UCC-psbD_p2	ATCAAACCGAAAGACCCCTT	GAAACGGTCCCTACGTAACC	45.0	55.0	57.0	58.0	1768-1795 bp	1.0	74.4
trnG-UCC-psbD_p3	GAATCAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	47.6	55.0	58.2	58.0	1770-1797 bp	1.0	72.5
trnG-UCC-psbD_p4	AATCAAACCGAAAGACCCCT	ACAAAAACGAAACGGTCCCT	45.0	45.0	57.0	58.2	1777-1804 bp	1.0	72.5
trnG-UCC-psbD_p5	ATCAAACCGAAAGACCCCTT	ACAAAAACGAAACGGTCCCT	45.0	45.0	57.0	58.2	1776-1803 bp	1.0	72.5