Hotspot

psbB

LSC · 72029-73555 · 1527 bp

Back to Leontodon

Pi 0.00164

MarkerSeek score 50.40

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 4
pi: 0.00164
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	ATCCACTAAAGAGGGGGTCC	TCCCGGATAAAGAAAGAGCA	55.0	45.0	58.1	56.2	1724-1736 bp	1.0	65.8
psbB_p2	ATCCACTAAAGAGGGGGTCC	TCCCGGATAAAGAAAGAGCAA	55.0	42.9	58.1	57.0	1724-1736 bp	1.0	64.0
psbB_p3	CCACTAAAGAGGGGGTCCAT	TCCCGGATAAAGAAAGAGCA	55.0	45.0	58.4	56.2	1722-1734 bp	1.0	63.4
psbB_p4	ATCCACTAAAGAGGGGGTCC	CCCGGATAAAGAAAGAGCAAGA	55.0	45.5	58.1	58.1	1723-1735 bp	1.0	62.9
psbB_p5	ATCCACTAAAGAGGGGGTCC	TCCCGGATAAAGAAAGAGCAAG	55.0	45.5	58.1	58.1	1724-1736 bp	1.0	62.9