Hotspot

petB

LSC · 74484-75880 · 1397 bp

Back to Leontodon

Pi 0.00179

MarkerSeek score 60.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1397
variable_sites: 4
pi: 0.00179
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CCCCCAAGTCCTAGCTTTTC	ATCGTTCCTACCCCAAGTGA	55.0	50.0	57.9	58.1	1565-1575 bp	1.0	79.5
petB_p2	CCCCCAAGTCCTAGCTTTTC	TCGTTCCTACCCCAAGTGAT	55.0	50.0	57.9	58.1	1564-1574 bp	1.0	79.5
petB_p3	TTTTGGTAGTTCGACCGTGG	ATCGTTCCTACCCCAAGTGA	50.0	50.0	58.1	58.1	1509-1513 bp	1.0	78.8
petB_p4	TTTTGGTAGTTCGACCGTGG	TCGTTCCTACCCCAAGTGAT	50.0	50.0	58.1	58.1	1508-1512 bp	1.0	78.8
petB_p5	GGTAGTTCGACCGTGGAATT	TCGTTCCTACCCCAAGTGAT	50.0	50.0	57.6	58.1	1504-1508 bp	1.0	76.8