Hotspot

clpP1

LSC · 69566-71573 · 2008 bp

Back to Leontodon

Pi 0.00324

MarkerSeek score 45.90

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2008
variable_sites: 10
pi: 0.00324
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	AAGTCGGCAGGGGTAATTTC	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2125-2133 bp	1.0	70.3
clpP1_p2	GAAAGTCGGCAGGGGTAATT	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2127-2135 bp	1.0	70.3
clpP1_p3	TATGAAAGTCGGCAGGGGTA	GGCTTTGTTACGTTTCCACA	50.0	45.0	58.1	56.9	2130-2138 bp	1.0	70.2
clpP1_p4	GTCGGCAGGGGTAATTTCAA	GGCTTTGTTACGTTTCCACA	50.0	45.0	58.2	56.9	2123-2131 bp	1.0	69.9
clpP1_p5	AAGTCGGCAGGGGTAATTTC	AGGCTTTGTTACGTTTCCACA	50.0	42.9	57.9	58.4	2126-2134 bp	1.0	68.6