Hotspot

rps15-ycf1

SSC · 122133-122531 · 399 bp

Back to Leontodon

Pi 0.0117
MarkerSeek score 31.00
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
399
variable_sites
7
pi
0.0117
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
31.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 ACTTGAAATTCAACAGATCCTCT CGCTTGAGTATGTTAAGGAT 34.8 40.0 55.8 52.8 518-523 bp 1.0 41.1
rps15-ycf1_p2 CAGATCCTCTGTTTTCTTTGT CGCTTGAGTATGTTAAGGAT 38.1 40.0 53.7 52.8 505-510 bp 1.0 41.1
rps15-ycf1_p3 AGTCTCCGTATCTTATTGGT CGCTTGAGTATGTTAAGGAT 40.0 40.0 52.9 52.8 548-553 bp 1.0 41.0
rps15-ycf1_p4 AAGTCTCCGTATCTTATTGGT CGCTTGAGTATGTTAAGGAT 38.1 40.0 53.8 52.8 549-554 bp 1.0 41.0
rps15-ycf1_p5 AGTAAGTCTCCGTATCTTATTGGT CGCTTGAGTATGTTAAGGAT 37.5 40.0 56.7 52.8 552-557 bp 1.0 41.0