Hotspot

ndhF-rpl32

SSC · 110880-111919 · 1040 bp

Back to Leontodon

Pi 0.00817

MarkerSeek score 50.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1040
variable_sites: 13
pi: 0.00817
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	ACAAAGCTTTTAACGCTGCC	50.0	45.0	58.0	57.9	1218-1235 bp	1.0	80.7
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	ACAAAGCTTTTAACGCTGCC	45.0	45.0	58.0	57.9	1216-1233 bp	1.0	80.6
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	ACAAAGCTTTTAACGCTGCC	45.0	45.0	58.0	57.9	1217-1234 bp	1.0	80.6
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	GCCCAATACCCCTTCCTTTT	50.0	50.0	58.0	57.8	1201-1218 bp	1.0	79.8
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	GCCCAATACCCCTTCCTTTT	45.0	50.0	58.0	57.8	1199-1216 bp	1.0	79.7