Hotspot

ycf4-cemA

LSC · 60116-60867 · 752 bp

Back to Leontodon

Pi 0.00953

MarkerSeek score 54.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 11
pi: 0.00953
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	932-934 bp	1.0	80.1
ycf4-cemA_p2	GAGCCATTCCATTGACTCGT	CCACCAATGAGTAACCCAGG	50.0	55.0	58.0	57.9	976-978 bp	1.0	79.6
ycf4-cemA_p3	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	896-898 bp	1.0	79.6
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	864-866 bp	1.0	79.6
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	CCACCAATGAGTAACCCAGG	45.0	55.0	58.1	57.9	908-910 bp	1.0	79.2