Pi
0.00291
MarkerSeek score
63.70
Primer available
yes
Label
ndhC-trnC-ACA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhC-trnC-ACA_p1 | CGGTCCTTTGTTACTCGGAG | CCGAGAAAGTCTACGGTTCG | 55.0 | 55.0 | 58.0 | 58.1 | 1281-1309 bp | 1.0 | 81.5 |
| ndhC-trnC-ACA_p2 | GGTCCTTTGTTACTCGGAGC | CCGAGAAAGTCTACGGTTCG | 55.0 | 55.0 | 58.3 | 58.1 | 1280-1308 bp | 1.0 | 79.3 |
| ndhC-trnC-ACA_p3 | ACTCGGAGCTAAAAATCCCG | CCGAGAAAGTCTACGGTTCG | 50.0 | 55.0 | 57.7 | 58.1 | 1269-1297 bp | 1.0 | 79.1 |
| ndhC-trnC-ACA_p4 | TTCCGGTCCTTTGTTACTCG | CCGAGAAAGTCTACGGTTCG | 50.0 | 55.0 | 57.6 | 58.1 | 1284-1312 bp | 1.0 | 78.0 |
| ndhC-trnC-ACA_p5 | CGGTCCTTTGTTACTCGGAG | GTTCGAGTCCGTATAGCCCT | 55.0 | 55.0 | 58.0 | 58.7 | 1266-1294 bp | 1.0 | 76.9 |