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trnE-UUC-rpoB

LSC · 12015-12951 · 937 bp

Back to Leontodon

Pi 0.00267

MarkerSeek score 46.20

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 937
variable_sites: 4
pi: 0.00267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.0	56.8	1078-1080 bp	1.0	62.8
trnE-UUC-rpoB_p2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.1	56.8	1037-1039 bp	1.0	62.1
trnE-UUC-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	1068-1070 bp	1.0	61.9
trnE-UUC-rpoB_p4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	1073-1075 bp	1.0	61.9
trnE-UUC-rpoB_p5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	50.0	40.9	58.0	57.6	1068-1070 bp	1.0	61.4