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psbM-trnD-GUC

LSC · 10918-11564 · 647 bp

Back to Leontodon

Pi 0.00489

MarkerSeek score 47.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 647
variable_sites: 5
pi: 0.00489
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACCAAAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	42.9	50.0	57.5	58.3	766 bp	1.0	66.8
psbM-trnD-GUC_p2	AGTAGAAAAGCGGTAGGAACC	TTCAATAGGCAAGAGCACCG	47.6	50.0	57.4	58.3	781 bp	1.0	65.6
psbM-trnD-GUC_p3	AGGAACCAAAATGAACAGTGC	TTCAATAGGCAAGAGCACCG	42.9	50.0	57.3	58.3	767 bp	1.0	64.6
psbM-trnD-GUC_p4	ACCAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	40.0	50.0	56.1	58.3	763 bp	1.0	62.9
psbM-trnD-GUC_p5	GAACCAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	40.9	50.0	58.0	58.3	765 bp	1.0	62.1