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trnK-UUU-rps16

LSC · 4384-5195 · 812 bp

Back to Leontodon

Pi 0.00246

MarkerSeek score 50.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 812
variable_sites: 3
pi: 0.00246
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	937-949 bp	1.0	77.4
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	936-948 bp	1.0	77.4
trnK-UUU-rps16_p3	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1021-1033 bp	1.0	76.8
trnK-UUU-rps16_p4	CAGTCGTGGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1020-1032 bp	1.0	76.7
trnK-UUU-rps16_p5	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	952-964 bp	1.0	76.6