Hotspot

ycf1

SSC · 126079-131601 · 5523 bp

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Pi 0.0102

MarkerSeek score 45.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5523
variable_sites: 56
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCTTGACATGCTGAAACGA	TAGCTATTTCGCAGGTGCAG	45.0	50.0	57.5	58.1	438 bp	1.0	76.8
ycf1_p2	CCTTGACATGCTGAAACGAC	TAGCTATTTCGCAGGTGCAG	50.0	50.0	57.4	58.1	437 bp	1.0	76.4
ycf1_p3	TCCTTGACATGCTGAAACGA	TTTCGCAGGTGCAGAAAAAC	45.0	45.0	57.5	57.8	432 bp	1.0	75.7
ycf1_p4	CCTTGACATGCTGAAACGAC	TTTCGCAGGTGCAGAAAAAC	50.0	45.0	57.4	57.8	431 bp	1.0	75.2
ycf1_p5	TCCTTGACATGCTGAAACGA	GCCGATTTCGAAGACCCTAA	45.0	50.0	57.5	57.7	551 bp	1.0	74.5