Hotspot

rpl22

LSC · 84884-85408 · 525 bp

Back to Leontice

Pi 0.0347

MarkerSeek score 73.80

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 525
variable_sites: 18
pi: 0.0347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GTTGTGCAAACCAAAGGGAA	TCCGCATTTATTGGTGGGAG	45.0	50.0	57.3	57.9	608-614 bp	1.0	77.3
rpl22_p2	GTTGTGCAAACCAAAGGGAA	GGGGCATGCGAGAAATGATA	45.0	50.0	57.3	58.1	673 bp	1.0	76.6
rpl22_p3	GTTGTGCAAACCAAAGGGAA	CGCATTTATTGGTGGGAGGT	45.0	50.0	57.3	58.2	606-612 bp	1.0	75.8
rpl22_p4	GTTGTGCAAACCAAAGGGAA	GGGAGAATTCGCACCTACTC	45.0	55.0	57.3	57.8	703 bp	1.0	75.5
rpl22_p5	TGTGCAAACCAAAGGGAATG	TCCGCATTTATTGGTGGGAG	45.0	50.0	57.1	57.9	606-612 bp	1.0	75.5