Hotspot

petB

LSC · 76156-77626 · 1471 bp

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Pi 0.00884

MarkerSeek score 63.90

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1471
variable_sites: 13
pi: 0.00884
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TCCGGGCTTTTCAATCCAAA	GTTCCTCCCCAAAATGATCGA	45.0	47.6	58.0	58.0	1630 bp	1.0	72.7
petB_p2	GGTCATTCTGGGAGTTCGAC	GTTCCTCCCCAAAATGATCGA	55.0	47.6	58.0	58.0	1583 bp	1.0	72.6
petB_p3	GTCATTCTGGGAGTTCGACC	GTTCCTCCCCAAAATGATCGA	55.0	47.6	58.0	58.0	1582 bp	1.0	72.6
petB_p4	AAGAATCATGAAGTCCGGGC	GTTCCTCCCCAAAATGATCGA	50.0	47.6	58.0	58.0	1643 bp	1.0	72.3
petB_p5	GAATCATGAAGTCCGGGCTT	GTTCCTCCCCAAAATGATCGA	50.0	47.6	58.0	58.0	1641 bp	1.0	72.3