Hotspot

ndhD

SSC · 117547-119133 · 1587 bp

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Pi 0.00819

MarkerSeek score 48.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1587
variable_sites: 13
pi: 0.00819
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCGAGATCCAAGACGTGAAT	GCATGAGACAACTCGTAGCA	50.0	50.0	58.1	58.0	2348-2384 bp	1.0	80.4
ndhD_p2	GCGAGATCCAAGACGTGAAT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.1	58.0	2437-2473 bp	1.0	80.3
ndhD_p3	CTAATGAGGCATGGGGATCG	GCATGAGACAACTCGTAGCA	55.0	50.0	57.9	58.0	2165-2210 bp	1.0	79.9
ndhD_p4	GCGAGATCCAAGACGTGAAT	GAGACAACTCGTAGCATGGG	50.0	55.0	58.1	58.1	2344-2380 bp	1.0	79.9
ndhD_p5	CTAATGAGGCATGGGGATCG	TCTGCTCCAAGAACAGAGGA	55.0	50.0	57.9	58.0	2254-2299 bp	1.0	79.8