Hotspot

ndhG-ndhI

SSC · 120860-121366 · 507 bp

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Pi 0.024

MarkerSeek score 63.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 507
variable_sites: 12
pi: 0.024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CGCCGACTATAAGACCCAAC	ACAATTGTGAATTCGACCCCA	55.0	42.9	58.2	58.1	626-631 bp	1.0	72.3
ndhG-ndhI_p2	GCCGACTATAAGACCCAACC	ACAATTGTGAATTCGACCCCA	55.0	42.9	57.8	58.1	625-630 bp	1.0	71.6
ndhG-ndhI_p3	TCCCAACGAAAAGGCAGAAT	ACAATTGTGAATTCGACCCCA	45.0	42.9	57.7	58.1	679-684 bp	1.0	71.0
ndhG-ndhI_p4	AATACTACTCCTAGCCCGCC	ACAATTGTGAATTCGACCCCA	55.0	42.9	58.4	58.1	642-647 bp	1.0	70.5
ndhG-ndhI_p5	CTAGCCCGCCGACTATAAGA	ACAATTGTGAATTCGACCCCA	55.0	42.9	58.5	58.1	632-637 bp	1.0	69.7