Hotspot

rpl32-trnL-UAG

SSC · 115197-116187 · 991 bp

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Pi 0.0188

MarkerSeek score 68.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 991
variable_sites: 18
pi: 0.0188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATCAAAGGGTATTGGGCAGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	1116-1117 bp	1.0	81.8
rpl32-trnL-UAG_p2	ATCAAAGGGTATTGGGCAGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	1117-1118 bp	1.0	81.8
rpl32-trnL-UAG_p3	ATCAAAGGGTATTGGGCAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	1126-1127 bp	1.0	80.9
rpl32-trnL-UAG_p4	ATCAAAGGGTATTGGGCAGC	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.2	57.8	1125-1126 bp	1.0	80.3
rpl32-trnL-UAG_p5	ATCAAAGGGTATTGGGCAGC	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.2	57.8	1124-1125 bp	1.0	80.3