Hotspot

psbT-psbN

LSC · 75506-75574 · 69 bp

Back to Leontice

Pi 0.058

MarkerSeek score 78.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 4
pi: 0.058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	ACGATCGGATTTATGGAAGCA	ACCCTAGTCGCCATCTTTCT	42.9	50.0	57.5	58.1	309-310 bp	1.0	68.2
psbT-psbN_p2	ACGATCGGATTTATGGAAGCA	GGGTACGCCTTATATACCGC	42.9	55.0	57.5	57.7	264-265 bp	1.0	67.2
psbT-psbN_p3	ACGATCGGATTTATGGAAGCA	GTTTTACCGGGTACGCCTTA	42.9	50.0	57.5	57.6	272-273 bp	1.0	66.4
psbT-psbN_p4	ACGATCGGATTTATGGAAGCA	TAAGTTTTACCGGGTACGCC	42.9	50.0	57.5	57.6	275-276 bp	1.0	66.4
psbT-psbN_p5	ACGATCGGATTTATGGAAGCA	GATCCATTCGAGGAACACGG	42.9	55.0	57.5	58.4	216-217 bp	1.0	66.3