Hotspot

trnF-GAA-ndhJ

LSC · 49889-50411 · 523 bp

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Pi 0.0154

MarkerSeek score 66.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 523
variable_sites: 8
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TCCACGCCTGAAACGTATTT	50.0	45.0	58.1	57.8	660-686 bp	1.0	79.4
trnF-GAA-ndhJ_p2	ATCGGGAAGAGTCGGGATAG	TCCACGCCTGAAACGTATTT	55.0	45.0	58.1	57.8	709-735 bp	1.0	79.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CATCCACGCCTGAAACGTAT	50.0	50.0	58.1	58.3	662-688 bp	1.0	78.0
trnF-GAA-ndhJ_p4	GGAATGATGCATCGGGAAGA	TCCACGCCTGAAACGTATTT	50.0	45.0	57.7	57.8	719-745 bp	1.0	77.6
trnF-GAA-ndhJ_p5	ATCGGGAAGAGTCGGGATAG	CATCCACGCCTGAAACGTAT	55.0	50.0	58.1	58.3	711-737 bp	1.0	77.6