Hotspot

psbZ-trnG-UCC

LSC · 37179-37692 · 514 bp

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Pi 0.0211

MarkerSeek score 58.30

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 514
variable_sites: 10
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TGGCTCAGAGAGGGATCAAA	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	1342-1348 bp	1.0	82.8
psbZ-trnG-UCC_p2	TTGGCTCAGAGAGGGATCAA	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	1343-1349 bp	1.0	82.8
psbZ-trnG-UCC_p3	TTTGGCTCAGAGAGGGATCA	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	1344-1350 bp	1.0	82.8
psbZ-trnG-UCC_p4	GCCAGGCGAAACAAGAAATC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	1452-1454 bp	1.0	82.6
psbZ-trnG-UCC_p5	GCCAGGCGAAACAAGAAATC	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	1774-1776 bp	1.0	82.3