Hotspot

trnC-GCA-petN

LSC · 28088-28755 · 668 bp

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Pi 0.012

MarkerSeek score 65.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 8
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	739-967 bp	1.0	80.2
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CGCTTCTTCCCCATACTACG	50.0	55.0	58.1	57.9	787-1015 bp	1.0	79.1
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	733-961 bp	1.0	78.8
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTAGAGTCCGCTTCTTCCC	50.0	55.0	58.1	58.2	796-1024 bp	1.0	78.3
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	757-985 bp	1.0	78.0