Hotspot

rpoB-trnC-GCA

LSC · 27202-28008 · 807 bp

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Pi 0.01

MarkerSeek score 65.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 8
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCCTCATTTGCATCTCGAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	893-894 bp	1.0	80.3
rpoB-trnC-GCA_p2	ATCCCCTCATTTGCATCTCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	895-896 bp	1.0	78.5
rpoB-trnC-GCA_p3	CCCCTCATTTGCATCTCGAA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	875-876 bp	1.0	78.2
rpoB-trnC-GCA_p4	GTTCTTCCATCAAGCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	972-973 bp	1.0	76.6
rpoB-trnC-GCA_p5	AGTTCTTCCATCAAGCCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	973-974 bp	1.0	76.5