Hotspot

atpH-atpI

LSC · 14109-15010 · 902 bp

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Pi 0.0136
MarkerSeek score 54.20
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
902
variable_sites
12
pi
0.0136
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 TCCAGGTCCAATAGAAGCCA GAATATGGTCCGTGGGTTCC 50.0 55.0 58.0 58.0 1454-1656 bp 1.0 81.0
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT GAATATGGTCCGTGGGTTCC 45.0 55.0 58.0 58.0 1411-1613 bp 1.0 81.0
atpH-atpI_p3 AGCAAGTGACAAGACCAAGG GAATATGGTCCGTGGGTTCC 50.0 55.0 58.0 58.0 1681-1883 bp 1.0 80.9
atpH-atpI_p4 CAGTGACCCAAGGAAACGAA GAATATGGTCCGTGGGTTCC 50.0 55.0 58.0 58.0 2125-2328 bp 1.0 80.8
atpH-atpI_p5 AGCAAGTGACAAGACCAAGG CTGTGCGAAATCCACAAACC 50.0 50.0 58.0 57.9 1779-1981 bp 1.0 80.7