Hotspot

atpF-atpH

LSC · 13404-13862 · 459 bp

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Pi 0.0196

MarkerSeek score 67.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 459
variable_sites: 9
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	AGTCGTGGCATTAGCACTTT	50.0	45.0	58.0	57.8	543-544 bp	1.0	79.5
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	ATTTATGGGCTAGTCGTGGC	50.0	50.0	58.0	57.7	554-555 bp	1.0	78.9
atpF-atpH_p3	TTAAATCCGAAACTCCCGGC	AGTCGTGGCATTAGCACTTT	50.0	45.0	58.3	57.8	570-571 bp	1.0	77.7
atpF-atpH_p4	TTAAATCCGAAACTCCCGGC	ATTTATGGGCTAGTCGTGGC	50.0	50.0	58.3	57.7	581-582 bp	1.0	77.1
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	CTAGTCGTGGCATTAGCACT	50.0	50.0	58.0	57.4	545-546 bp	1.0	76.3