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trnK-UUU-rps16

LSC · 4741-5748 · 1008 bp

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Pi 0.0176

MarkerSeek score 68.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1008
variable_sites: 14
pi: 0.0176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	913-1122 bp	1.0	83.6
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	912-1121 bp	1.0	83.6
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	TCAAATGTTCCCGCGATTCT	50.0	45.0	58.2	57.9	944-1153 bp	1.0	82.9
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TCAAATGTTCCCGCGATTCT	50.0	45.0	58.2	57.9	943-1152 bp	1.0	82.9
trnK-UUU-rps16_p5	TCTAGCCGCACTTAAAAGCC	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	927-1136 bp	1.0	82.8