Hotspot

trnH-GUG-psbA

LSC · 193-869 · 677 bp

Back to Leontice

Pi 0.00904

MarkerSeek score 56.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 5
pi: 0.00904
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	690-801 bp	1.0	83.2
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.2	58.0	691-802 bp	1.0	83.0
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	TATCATCAACCGCGCTAACC	50.0	50.0	57.9	58.1	701-812 bp	1.0	82.3
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	TATCATCAACCGCGCTAACC	50.0	50.0	58.2	58.1	702-813 bp	1.0	82.1
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	CTGATATCATCAACCGCGCT	50.0	50.0	57.9	58.2	705-816 bp	1.0	81.8