Hotspot

ndhF

SSC · 121244-123472 · 2229 bp

Pi 0.0188

MarkerSeek score 53.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 42
pi: 0.0188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CAGTTGGATATTCGGGAGCC	AGCCTAGTCACCGGAACTTA	55.0	50.0	58.1	57.8	2849-2870 bp	1.0	80.0
ndhF_p2	CAGTTGGATATTCGGGAGCC	TATCTGAGCCTAGTCACCGG	55.0	55.0	58.1	58.0	2855 bp	0.5	58.9
ndhF_p3	GTTGGATATTCGGGAGCCAA	TATCTGAGCCTAGTCACCGG	50.0	55.0	57.7	58.0	2853 bp	0.5	57.1
ndhF_p4	TGGATATGACTGGACCGACT	TATCTGAGCCTAGTCACCGG	50.0	55.0	57.5	58.0	2671 bp	0.5	56.2
ndhF_p5	CAGTTGGATATTCGGGAGCC	TCCTTCCGAACCGAAAGAGA	55.0	50.0	58.1	58.4	2655 bp	0.5	56.1