Hotspot

ndhD-psaC

SSC · 128014-128152 · 139 bp

Back to Lemna

Pi 0.0455
MarkerSeek score 70.40
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
139
variable_sites
6
pi
0.0455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 GAAACAGCCCGCAAATATCG TCGAAGTATGGGCCTAGGTT 50.0 50.0 57.9 57.8 217-224 bp 1.0 82.5
ndhD-psaC_p2 GAAACAGCCCGCAAATATCG ACTCGAAGTATGGGCCTAGG 50.0 55.0 57.9 58.3 219-226 bp 1.0 81.5
ndhD-psaC_p3 GAAACAGCCCGCAAATATCG CTCGAAGTATGGGCCTAGGT 50.0 55.0 57.9 58.3 218-225 bp 1.0 81.5
ndhD-psaC_p4 AAAATGAAACAGCCCGCAAA TCGAAGTATGGGCCTAGGTT 40.0 50.0 57.4 57.8 222-229 bp 1.0 78.4
ndhD-psaC_p5 AAAAATGAAACAGCCCGCAA TCGAAGTATGGGCCTAGGTT 40.0 50.0 57.4 57.8 223-230 bp 1.0 78.3