Hotspot

ndhF-rpl32

SSC · 123473-124542 · 1070 bp

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Pi 0.0415
MarkerSeek score 76.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1070
variable_sites
43
pi
0.0415
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CAGGTAGTGGAAGGAGAGGT TGACATAGAAGTGCGTTTTT 55.0 35.0 57.8 53.5 1149-1155 bp 1.0 47.2
ndhF-rpl32_p2 CAGGTAGTGGAAGGAGAGGT TTGACATAGAAGTGCGTTTT 55.0 35.0 57.8 53.5 1150-1156 bp 1.0 47.2
ndhF-rpl32_p3 CAGGTAGTGGAAGGAGAGGT TTTGACATAGAAGTGCGTTT 55.0 35.0 57.8 53.5 1151-1157 bp 1.0 47.2
ndhF-rpl32_p4 CAGGTAGTGGAAGGAGAGGT TTTTGACATAGAAGTGCGTT 55.0 35.0 57.8 53.5 1152-1158 bp 1.0 47.2
ndhF-rpl32_p5 CAGGTAGTGGAAGGAGAGGT TTTTTGACATAGAAGTGCGT 55.0 35.0 57.8 53.5 1153-1159 bp 1.0 47.2