Hotspot

petA-psbJ

LSC · 67854-68976 · 1123 bp

Back to Lemna

Pi 0.0255
MarkerSeek score 75.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1123
variable_sites
28
pi
0.0255
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GTCACGTGTCCAAGGTCTTT TCCGGATTAGGCTCATCTCT 50.0 50.0 58.1 57.3 1256-1262 bp 1.0 77.4
petA-psbJ_p2 GTCACGTGTCCAAGGTCTTT CCGGATTAGGCTCATCTCTG 50.0 55.0 58.1 57.3 1255-1261 bp 1.0 76.9
petA-psbJ_p3 GTCACGTGTCCAAGGTCTTT TTCCGGATTAGGCTCATCTCT 50.0 47.6 58.1 58.0 1257-1263 bp 1.0 74.5
petA-psbJ_p4 ACGTGTCCAAGGTCTTTTGT TCCGGATTAGGCTCATCTCT 45.0 50.0 57.6 57.3 1253-1259 bp 1.0 74.5
petA-psbJ_p5 ACGTGTCCAAGGTCTTTTGT CCGGATTAGGCTCATCTCTG 45.0 55.0 57.6 57.3 1252-1258 bp 1.0 74.0