Hotspot

tRNA-Glu(UUC)-tRNA-Thr(GGU)

LSC · 33996-34870 · 875 bp

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Pi 0.0358
MarkerSeek score 75.30
Primer available yes
Label tRNA-Glu(UUC)-tRNA-Thr(GGU)

Feature Metadata

label_name
tRNA-Glu(UUC)-tRNA-Thr(GGU)
feature_type
igs
region
LSC
length_bp
875
variable_sites
30
pi
0.0358
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p1 ACTTTCCCAGTACCCTACCC GAACCGATGACTTACGCCTT 55.0 50.0 58.0 58.0 1018-1404 bp 1.0 84.1
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p2 ACTTTCCCAGTACCCTACCC CCATGGCGTTACTCTACCAC 55.0 55.0 58.0 58.1 997-1383 bp 1.0 83.8
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p3 ACTTTCCCAGTACCCTACCC TGGCGTTACTCTACCACTGA 55.0 50.0 58.0 58.1 994-1380 bp 1.0 83.6
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p4 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 962-1348 bp 1.0 83.5
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p5 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 960-1346 bp 1.0 83.5