Hotspot

rps16

LSC · 2407-3451 · 1045 bp

Back to Lemmaphyllum

Pi 0.00191

MarkerSeek score 53.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1045
variable_sites: 2
pi: 0.00191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GAATCTGTAAACTGGGCCCA	TTTCGCGTCAGAAAGTTTGC	50.0	45.0	57.5	57.9	1161-1163 bp	1.0	75.3
rps16_p2	GAATCTGTAAACTGGGCCCA	GCGTCAGAAAGTTTGCACAA	50.0	45.0	57.5	57.8	1157-1159 bp	1.0	74.6
rps16_p3	GGAACGACAGATACGGCATA	TTTCGCGTCAGAAAGTTTGC	50.0	45.0	57.2	57.9	1229-1231 bp	1.0	73.0
rps16_p4	AGAATCTGTAAACTGGGCCC	TTTCGCGTCAGAAAGTTTGC	50.0	45.0	57.2	57.9	1162-1164 bp	1.0	72.9
rps16_p5	GGAACGACAGATACGGCATA	GCGTCAGAAAGTTTGCACAA	50.0	45.0	57.2	57.8	1225-1227 bp	1.0	72.3