Hotspot

atpA

LSC · 8822-10345 · 1524 bp

Back to Lemmaphyllum

Pi 0.000656

MarkerSeek score 59.10

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 1
pi: 0.000656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TGGATAAGGCACGAGGGTTA	TTTTCATCTCATCACGCGGG	50.0	50.0	58.1	58.6	1644 bp	1.0	73.9
atpA_p2	ATTGGGCGTAATTCCGTGTT	TTTTCATCTCATCACGCGGG	45.0	50.0	58.2	58.6	1702 bp	1.0	73.6
atpA_p3	TGGATAAGGCACGAGGGTTA	ATCTCATCACGCGGGTTTTA	50.0	45.0	58.1	57.3	1639 bp	1.0	73.5
atpA_p4	TGGATAAGGCACGAGGGTTA	TCTCATCACGCGGGTTTTAT	50.0	45.0	58.1	57.3	1638 bp	1.0	73.5
atpA_p5	ATTGGGCGTAATTCCGTGTT	TCTCATCACGCGGGTTTTAT	45.0	45.0	58.2	57.3	1696 bp	1.0	73.2