Hotspot

ccsA-ndhD

SSC · 114051-114467 · 417 bp

Back to Lemmaphyllum

Pi 0.00242

MarkerSeek score 50.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 417
variable_sites: 1
pi: 0.00242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGGAGTCAATTTGTTGGGG	TGCTTTCTTATTCGGCTACCA	50.0	42.9	58.0	57.4	493-496 bp	1.0	67.6
ccsA-ndhD_p2	GGGAGTCAATTTGTTGGGGA	TGCTTTCTTATTCGGCTACCA	50.0	42.9	57.7	57.4	492-495 bp	1.0	65.3
ccsA-ndhD_p3	TGGGAGTCAATTTGTTGGGG	GCTTTCTTATTCGGCTACCAGA	50.0	45.5	58.0	58.2	492-495 bp	1.0	62.9
ccsA-ndhD_p4	TGGGAGTCAATTTGTTGGGG	CTGCTTTCTTATTCGGCTACC	50.0	47.6	58.0	56.8	494-497 bp	1.0	62.8
ccsA-ndhD_p5	TGGGAGTCAATTTGTTGGGG	GCTTTCTTATTCGGCTACCAG	50.0	47.6	58.0	56.8	492-495 bp	1.0	62.8