Hotspot

trnT-UGU-trnR-ACG

IRb · 84230-84965 · 736 bp

Back to Lemmaphyllum

Pi 0.00136

MarkerSeek score 59.50

Primer available yes

Label trnT-UGU-trnR-ACG

Feature Metadata

label_name: trnT-UGU-trnR-ACG
feature_type: igs
region: IRb
length_bp: 736
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnR-ACG_p1	TGCCTCAAAAGAGGGGTAGA	TGAAACGGATCCAGTAGGGT	50.0	50.0	58.0	58.1	913 bp	1.0	79.4
trnT-UGU-trnR-ACG_p2	AGTACGGAGGTTCGAATCCT	TGAAACGGATCCAGTAGGGT	50.0	50.0	57.9	58.1	864 bp	1.0	78.5
trnT-UGU-trnR-ACG_p3	TTTCCCTGCCTCAAAAGAGG	TGAAACGGATCCAGTAGGGT	50.0	50.0	57.7	58.1	919 bp	1.0	77.3
trnT-UGU-trnR-ACG_p4	GGATTGGTGAGCGTAACTCC	TGAAACGGATCCAGTAGGGT	55.0	50.0	58.3	58.1	945 bp	1.0	76.9
trnT-UGU-trnR-ACG_p5	CGTTTCCCTGCCTCAAAAGA	TGAAACGGATCCAGTAGGGT	50.0	50.0	58.4	58.1	921 bp	1.0	76.5