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psbK-psbI

LSC · 5944-6464 · 521 bp

Back to Lemmaphyllum

Pi 0.00385

MarkerSeek score 59.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 2
pi: 0.00385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	TGCCAAATTACCCGAAGCTT	ATCTAATCCCTACGTCCGGG	45.0	55.0	58.1	58.1	752-754 bp	1.0	79.0
psbK-psbI_p2	TTGCCAAATTACCCGAAGCT	ATCTAATCCCTACGTCCGGG	45.0	55.0	58.1	58.1	753-755 bp	1.0	79.0
psbK-psbI_p3	TGCCAAATTACCCGAAGCTT	GGGTTTCGTCCAGGATCATT	45.0	50.0	58.1	57.6	734-736 bp	1.0	76.3
psbK-psbI_p4	TTGCCAAATTACCCGAAGCT	GGGTTTCGTCCAGGATCATT	45.0	50.0	58.1	57.6	735-737 bp	1.0	76.3
psbK-psbI_p5	GCCGCAGTTAGCTTTCGATA	ATCTAATCCCTACGTCCGGG	50.0	55.0	58.4	58.1	655-657 bp	1.0	76.3