Hotspot

rps15

SSC · 123479-123745 · 267 bp

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Pi 0

MarkerSeek score 23.50

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 267
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TCTGTGCACCAATATCTGCC	TTCTACCCCCTAAACGACGT	50.0	50.0	58.0	58.1	1436-1437 bp	1.0	79.3
rps15_p2	GCTGGCCCTTTTAGGAACTT	TTCTACCCCCTAAACGACGT	50.0	50.0	58.1	58.1	1348-1349 bp	1.0	79.0
rps15_p3	TCTGTGCACCAATATCTGCC	TCAAAACCGGACCACAGTTT	50.0	45.0	58.0	57.9	1336-1337 bp	1.0	78.8
rps15_p4	TCTGTGCACCAATATCTGCC	TTCAAAACCGGACCACAGTT	50.0	45.0	58.0	57.9	1337-1338 bp	1.0	78.8
rps15_p5	GCTGGCCCTTTTAGGAACTT	TCAAAACCGGACCACAGTTT	50.0	45.0	58.1	57.9	1248-1249 bp	1.0	78.6