Hotspot

rpoA

LSC · 77670-78653 · 984 bp

Back to Leiospora

Pi 0.00203

MarkerSeek score 59.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 984
variable_sites: 2
pi: 0.00203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	ACGTTGCAGGTTTAAAATCCG	TAAAGGCTGAAAGGGTTTCA	42.9	40.0	57.7	54.8	1117 bp	1.0	43.9
rpoA_p2	CGTTGCAGGTTTAAAATCCGT	TAAAGGCTGAAAGGGTTTCA	42.9	40.0	57.7	54.8	1116 bp	1.0	43.9
rpoA_p3	ACGTTGCAGGTTTAAAATCCG	GGTTGTAGACCCCCTAAAAA	42.9	45.0	57.7	54.5	1157 bp	1.0	42.2
rpoA_p4	CGTTGCAGGTTTAAAATCCGT	GGTTGTAGACCCCCTAAAAA	42.9	45.0	57.7	54.5	1156 bp	1.0	42.2
rpoA_p5	CGTTGCAGGTTTAAAATCCGT	ATAAAGGCTGAAAGGGTTTCA	42.9	38.1	57.7	55.4	1117 bp	1.0	40.9