Hotspot

rpl16

LSC · 80953-82457 · 1505 bp

Back to Leiospora

Pi 0.00133

MarkerSeek score 59.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1505
variable_sites: 2
pi: 0.00133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTTTTAATGCAAAGGACGAAGT	ATTTCCATCAAAACTCCTTTG	34.8	33.3	57.0	52.5	1639-1641 bp	1.0	40.3
rpl16_p2	TCTTTTAATGCAAAGGACGAAGT	AATTTCCATCAAAACTCCTTTG	34.8	31.8	57.0	53.4	1640-1642 bp	1.0	40.3
rpl16_p3	CTCTTCTTTTAATGCAAAGGACGA	ATTTCCATCAAAACTCCTTTG	37.5	33.3	57.8	52.5	1643-1645 bp	1.0	40.3
rpl16_p4	TCTCTTCTTTTAATGCAAAGGACG	ATTTCCATCAAAACTCCTTTG	37.5	33.3	57.8	52.5	1644-1646 bp	1.0	40.3
rpl16_p5	TCTTCTTTTAATGCAAAGGACGA	ATTTCCATCAAAACTCCTTTG	34.8	33.3	56.7	52.5	1642-1644 bp	1.0	40.3