Hotspot

ycf3-trnS-UCC

LSC · 43451-44533 · 1083 bp

Back to Leiospora

Pi 0.00185

MarkerSeek score 59.80

Primer available yes

Label ycf3-trnS-UCC

Feature Metadata

label_name: ycf3-trnS-UCC
feature_type: igs
region: LSC
length_bp: 1083
variable_sites: 2
pi: 0.00185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-UCC_p1	GGGTCAATTTCTAGGCGCAT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.3	58.1	1227 bp	1.0	76.8
ycf3-trnS-UCC_p2	CCTTCAGATTGAGCCGACAT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.7	58.1	1170 bp	1.0	76.8
ycf3-trnS-UCC_p3	GGGTCAATTTCTAGGCGCAT	TGTTACGCCTTCAACCACTC	50.0	50.0	58.3	58.1	1192 bp	1.0	76.6
ycf3-trnS-UCC_p4	CCTTCAGATTGAGCCGACAT	TGTTACGCCTTCAACCACTC	50.0	50.0	57.7	58.1	1135 bp	1.0	76.6
ycf3-trnS-UCC_p5	GGGTCAATTTCTAGGCGCAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	1248 bp	1.0	76.4