Hotspot

petN-psbM

LSC · 28155-28705 · 551 bp

Back to Leiospora

Pi 0.00364

MarkerSeek score 60.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 551
variable_sites: 2
pi: 0.00364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGCTGCGTTAATGGTAGTT	CGTTCCTACTGCCTTTTTGC	50.0	50.0	58.3	57.7	676-677 bp	1.0	75.7
petN-psbM_p2	TAGTGTGGGGAAGAAGTGGA	CGTTCCTACTGCCTTTTTGC	50.0	50.0	57.6	57.7	634-635 bp	1.0	74.5
petN-psbM_p3	GTAGTGTGGGGAAGAAGTGG	CGTTCCTACTGCCTTTTTGC	55.0	50.0	57.5	57.7	635-636 bp	1.0	73.9
petN-psbM_p4	GTGGGGAAGAAGTGGACTCT	CGTTCCTACTGCCTTTTTGC	55.0	50.0	58.6	57.7	630-631 bp	1.0	72.6
petN-psbM_p5	GGGGACAGAATTCACATGGA	CGTTCCTACTGCCTTTTTGC	50.0	50.0	57.2	57.7	713-714 bp	1.0	71.2