Hotspot

rpoB-trnC-UGC

LSC · 26232-27337 · 1106 bp

Back to Leiospora

Pi 0.00273

MarkerSeek score 61.10

Primer available yes

Label rpoB-trnC-UGC

Feature Metadata

label_name: rpoB-trnC-UGC
feature_type: igs
region: LSC
length_bp: 1106
variable_sites: 3
pi: 0.00273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-UGC_p1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1185-1194 bp	1.0	79.0
rpoB-trnC-UGC_p2	GTTCCCTCTTTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	58.4	1167-1176 bp	1.0	76.8
rpoB-trnC-UGC_p3	GTTCCCTCTTTTCCATCCCC	TCAAGTCTGTTGTTGAGGCG	55.0	50.0	57.9	58.4	1209-1218 bp	1.0	76.3
rpoB-trnC-UGC_p4	TGTTCCCTCTTTTCCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.1	58.1	1186-1195 bp	1.0	73.0
rpoB-trnC-UGC_p5	GTTCCCTCTTTTCCATCCCC	AAGTCTGTTGTTGAGGCGG	55.0	52.6	57.9	58.0	1207-1216 bp	1.0	71.6