Hotspot

trnR-AGA-atpA

LSC · 9519-9826 · 308 bp

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Pi 0.00649

MarkerSeek score 42.30

Primer available yes

Label trnR-AGA-atpA

Feature Metadata

label_name: trnR-AGA-atpA
feature_type: igs
region: LSC
length_bp: 308
variable_sites: 2
pi: 0.00649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-AGA-atpA_p1	AGCCTTCCAAGCTAACGATG	CCAGTCAGGGGATGTTTCAG	50.0	55.0	58.0	57.9	1144 bp	1.0	79.0
trnR-AGA-atpA_p2	TTCTGTGAAAGCACCCAGAC	CCAGTCAGGGGATGTTTCAG	50.0	55.0	58.0	57.9	1345 bp	1.0	79.0
trnR-AGA-atpA_p3	ACCCTTAGCCTTCCAAGCTA	CCAGTCAGGGGATGTTTCAG	50.0	55.0	58.0	57.9	1150 bp	1.0	79.0
trnR-AGA-atpA_p4	CGACGTCGACTAAAACCCTT	CCAGTCAGGGGATGTTTCAG	50.0	55.0	57.9	57.9	1164 bp	1.0	78.8
trnR-AGA-atpA_p5	AAACCCTTAGCCTTCCAAGC	CCAGTCAGGGGATGTTTCAG	50.0	55.0	58.1	57.9	1152 bp	1.0	78.6