Hotspot

trnS-AGC-trnG-GGA

LSC · 7879-8494 · 616 bp

Back to Leiospora

Pi 0.00327

MarkerSeek score 61.30

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 616
variable_sites: 2
pi: 0.00327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	GGAACGAATCACACTTTTACCA	50.0	40.9	57.8	56.9	684-718 bp	1.0	54.7
trnS-AGC-trnG-GGA_p2	AACGCTTTAGTCCACTCAGC	GGAACGAATCACACTTTTACCA	50.0	40.9	58.2	56.9	682-716 bp	1.0	54.4
trnS-AGC-trnG-GGA_p3	CCAACGCTTTAGTCCACTCA	AGGGATTAAAGGAACGAATCACA	50.0	39.1	57.8	57.8	694-728 bp	1.0	54.0
trnS-AGC-trnG-GGA_p4	AACGCTTTAGTCCACTCAGC	AGGGATTAAAGGAACGAATCACA	50.0	39.1	58.2	57.8	692-726 bp	1.0	53.7
trnS-AGC-trnG-GGA_p5	CCAACGCTTTAGTCCACTCA	GGAACGAATCACACTTTTACCAC	50.0	43.5	57.8	58.2	684-718 bp	1.0	53.6