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trnK-AAA-rps16

LSC · 4393-5084 · 692 bp

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Pi 0.00292

MarkerSeek score 61.30

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 2
pi: 0.00292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	AAGCCGAGTACTCTACCGTT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	782-788 bp	1.0	76.4
trnK-AAA-rps16_p2	AAAGCCGAGTACTCTACCGT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	783-789 bp	1.0	76.3
trnK-AAA-rps16_p3	TACCGTTGAGTTAGCAACCC	CGCTCAACCAACAAGAACAG	50.0	50.0	57.5	57.6	769-775 bp	1.0	74.0
trnK-AAA-rps16_p4	AGTCGCACTTAAAAGCCGAG	CGCTCAACCAACAAGAACAG	50.0	50.0	58.6	57.6	794-800 bp	1.0	73.1
trnK-AAA-rps16_p5	AAGCCGAGTACTCTACCGTT	AGGCAGGGATTTTTACGGAA	50.0	45.0	58.2	57.1	744-750 bp	1.0	72.4