Hotspot

psaC

SSC · 115070-115315 · 246 bp

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Pi 0.497

MarkerSeek score 71.40

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 90
pi: 0.497
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	AGAAACCCGTGCTCCAAAAT	AAAACTCGGGGAACTCATCG	45.0	50.0	58.0	57.9	438 bp	0.5	59.2
psaC_p2	CTCCCGCCGTGTACAAAATA	AAAACTCGGGGAACTCATCG	50.0	50.0	58.0	57.9	985 bp	0.5	59.1
psaC_p3	TCTTTCCTCCCCACATGGAT	AAAACTCGGGGAACTCATCG	50.0	50.0	58.0	57.9	940 bp	0.5	59.1
psaC_p4	AGAAACCCGTGCTCCAAAAT	CTCGGGGAACTCATCGATTC	45.0	55.0	58.0	57.9	434 bp	0.5	58.8
psaC_p5	CTCCCGCCGTGTACAAAATA	CTCGGGGAACTCATCGATTC	50.0	55.0	58.0	57.9	981 bp	0.5	58.8